Related pages:
Chemical Safety Levels Table	Requirements for all Chemical Work	CSL 2 Requirements	CSL 3 Requirements	CSL 4 Requirements

Special Safety Practices for CSL3

1. Access to CSL3 laboratories is restricted to authorized personnel at all times. Visitors must obtain permission from the laboratory supervisor or other organizational management officials before escorted entry into these laboratories. The management of each organization may establish its own policy for visitors.

2. Work with materials with NFPA hazard ratings of 2 or greater in any category shall be performed in a properly functioning fume hood that is vented to outside the building.

3. Appropriate personal protective equipment shall be specific for the hazard of the chemicals. Safety glasses with side shields are required in laboratories where chemicals are used or stored. Wearing contact lenses in the laboratory is not recommended, but if an individual must wear them, they must also wear safety glasses with side shields or chemical splash goggles at all times in the laboratory. Protective gloves shall be selected on the basis of the chemicals being used. NOTE THERE IS NO UNIVERSAL PROTECTIVE GLOVE FOR ALL PURPOSES. FOR THE SAFETY OF PERSONNEL GLOVES MUST BE CHEMICAL/PURPOSE SPECIFIC.

4. All chemicals must be stored properly, according to compatibility. Any chemicals which pose hazard or risk shall be limited to the minimum quantities required to meet short-term needs of the research program. Chemicals not in use shall be stored in proper storage cabinets and not in chemical fume hoods. Regulated chemicals, such as drugs or radioactive materials, require special secured storage areas. Do not store chemicals on floors.

5. All chemical wastes shall be identified on a chemical waste label as to content and shall be placed in appropriate containers for disposal through the chemical waste disposal program. Any wastes which pose a special hazard shall be so identified and isolated for special handling.

6. Planning shall be guided by these principles:
i) be sure that the chemical with its inherit hazards can be used safely in your laboratory, that is, you have the proper facility and available resources to safely carry out this work;
ii) whenever possible substitute less toxic alternatives for more highly hazardous chemicals;
iii) use the smallest quantity of chemical that is practical for the conduct of the experiment; and iv) purchase the minimum quantity needed to conduct all experiments. Disposal is not trivial and generally costs 3 to 10 times the original cost of a chemical. This is a hidden cost when you purchase a chemical.

Special Safety Equipment for CSL3

1. Any refrigerators or freezers used for storage of chemicals or samples containing chemicals shall be rated as laboratory-safe or explosion-proof.

2. A vented flammable material storage cabinet, either built in or free- standing, shall be used for the storage of flammable materials. The cabinets must meet the minimum OSHA standards described in 29 CFR 1910.106.

3. The laboratory must be equipped with a vented fume hood. Ductless fume hoods are not acceptable. The fume hood shall be certified to meet the minimum performance standards given in ANSI Z9.5 and ASHRAE 110-1995.

4. Emergency eye washes shall be provided in each laboratory that uses chemicals and shall be tested weekly by laboratory personnel. Emergency showers shall be within 10 seconds walking distance or 100 feet of the laboratory. Emergency showers shall be tested at least annually by the maintenance department. Eye washes and showers must follow ANSI Z358.1 performance standards. The plumbing shall be sized to accommodate the deluge shower and the eyewash station.

5. The laboratory shall have a balance that is ventilated to provide protection from vapors, fumes, dusts, or aerosols.

6. Hot plates or other heating devices used for heating solutions containing flammable chemicals must be spark-proof.

7. Carts or safety carriers of rubber or polyethylene for the transport of bottles of acids, bases, solvents, or other hazardous chemicals shall be provided by each program and used by all personnel in transporting these chemicals between storage areas and laboratories.

8. Centrifuges shall be equipped with rotors which have sealed buckets or capped/shielded tubes to prevent aerosol formation in the event of a broken or cracked tube.

9. Spill control kits shall be available and personnel shall be trained in their use. Any spills or accidents will be promptly cleaned up.

10. Each laboratory shall be equipped with one or more fire extinguisher that meets that laboratory’s specific requirements.

Special Laboratory Facility Requirements for CSL3

1.Two well-separated exit doors should be available to the laboratory. The requirements for a second exit are described in NFPA 45 Standard.

2.The facility should include a separation of work spaces and desk areas.

3.The ventilation system shall be designed such that the room air balance is maintained at a negative pressure with respect to the corridors whether the fume hood is on or off.

4.Local exhaust ventilation will be available if needed to remove toxic vapors, fumes, dusts, particulates, or other aerosols.