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GENERAL SURVEY OF LABORATORIES IN DIFFERENT BUILDINGS ACROSS UVM CAMPUS
(This is by no means an exhaustive list. It is only being used to gain an idea of current processes of some laboratories around campus.)

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TOM TEEL (PATHOLOGY IN GIVEN BUILIDING) 6-0377

• numerous glass pipets from five labs
  
• non biohazards are wasted daily in a special washing machine
  
• cheap anionic dishwashing detergent used (unsure of specific name)
  
• material being washed is liquid tissue culture media
  
• methodology used because it has been established for a long time.
  
• biohazards are autoclaved and then disposed
  
• numerous quantities of biohazard and non biohazard liquid used in pipets everyday
  

  ADAM (LAB TECH IN AGRI TESTING LAB AT HILLS BUILDING) 6-3030

• about 30 beakers and volumetric flasks are used daily
  
• beakers contain concentrated nitric and perchlorate acid
  
• nitric and perchloric then diluted into volumetrics
  
• empty beakers are washed daily/ volumetrics washed weekly
  
• volumetrics and beakers are washed with tap and distilled water
  
• diluted nitric acid is poured down the drain, not neutralized
  
• methodology is research specific, must avoid elemental contamination
  
• have microwave but is not used because of faulty hood
  

  DON TOBI(FORESTRY RESEARCH AT AIKEN SNR) 6-3269

• volume of glassware used depends on year, most in summer
  
• glassware washed weekly
  
• about 200 glass items washed
  
• 20 Liters HCL 20% solution used and stored in fume hood for reuse
  
• HCL lasts for 3/4 months
  
• once volume of HCL reaches around 5L it is packed and given to ESF
  
• methodology is research specific, must guard against traces of phosphorus
  

  BRENDA TESSMAN (MMG IN STAFFORD BUILDING) 6-8254

• all biohazardous material is autoclaved and disposed
  
• non biohazard glassware is pre-rinsed with detergent and bleach
  
• pre-rinse water is drained in sink when done
  
• after pre-rinse glassware is picked up in loads and washed in a "dishwasher"
  
• AMSCO brand "dishwasher" (hot lab #1021)
  
• AMSCO brand detergent (#470)
  
• contact Louise Brown in Preperation Room Spec MMG Stafford (61125)
  

  VILLA RAKOCHY (BIO DEPARTMENT) 6-0704

• 20-30 pieces of glassware are used and washed daily
  
• glassware used is either reusable or disposable
  
• salt and glycerol residue washed out of some glassware
  
• chemical waste includes alcohol, DMSO, and acids
  
• chemicals with trace concentrations are poured down the sink.
  
• chemicals with stronger concentrations are put in a designated waste container
  
• waste container is picked up by ESF when full
  
• pasture pipettes and graduated pipettes are acid washed due to small diameter
  
• 8L 10% sulfuric acid batch is used every 2/3 months. picked up by ESF when unusable
  
• after acid wash, pipettes are washed in a 4 hour cycling Nalgene pipette washer which is piped into regular plumbing. this is to get rid of sulfuric acid left over.
  
• all other glassware is washed with MICROSOAP

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  Document Information: Version .07 by Nicholas Shih, Last Update: February 26, 1999